The Fluorescence Guide: Understand Fluorescence And How It Enhances Scientific Exploration

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Fluorophores and fluorescence detection play a key role in many research applications. Immunofluorescence and fluorescent proteins are relied upon for numerous fixed and live cell imaging techniques. Fluorescence-based methods are also widely used in laboratory assays such as flow cytometry, ELISA, western blot, immunohistochemistry (IHC), and immunocytochemistry (ICC). Fluorescent detection techniques offer several advantages, including high sensitivity, quantitative analysis, and the ability to perform multiplex analysis using various lasers, optical filters, and fluorophores.

Deepen your understanding of fluorescence for research, and gain valuable insight into how to select appropriate fluorophores and fluorescent proteins for your experiments. This guide introduces the fundamentals of fluorescence and covers advanced fluorescence mechanisms and essential reagents for fluorescence-based research.