TaqPath qPCR And RT-qPCR Master Mixes

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With superior quality, excellent performance, and regulatory compliance, Applied Biosystems TaqPath master mixes are designed to deliver confidence and performance for even your most demanding applications. With over 15 years of technology leadership in real-time PCR, we are committed to providing clinical laboratories with trusted, versatile, and innovative tools for molecular diagnostic development and testing.

Enabling confidence and compliance for diagnostic development and testing

TaqPath qPCR products

TaqPath qPCR master mixes have been validated to deliver exquisite lot-to-lot reproducibility for absolute Ct consistency and dynamic range across a wide variety of assays and applications. These general purpose reagents are manufactured in an ISO 13485 facility and include control measures, change control, document control, and purchasing controls. In addition, each batch is quality control–tested prior to release. TaqPath qPCR master mixes enable superior performance, improved reproducibility, and traceability.

Compatible with both standard and fast cycling modes, Applied Biosystems TaqPath qPCR Master Mixes have been engineered for enhanced stability, allowing your preassembled reactions to be left at room temperature for more than 24 hours without impacting performance. In addition, this product has been rigorously validated for duplex performance with both endogenous and exogenous controls, and each manufacturing lot is tested for duplex performance.

High performance

Through extensive benchmarking of this formulation against other leading suppliers’ standard and fast master mixes, TaqPath qPCR and RT-qPCR Master Mix has demonstrated the ability to deliver superior sensitivity, accuracy, dynamic range, and specificity.

TaqPath 1-Step Multiplex Master Mix

TaqPath 1-Step Multiplex Master Mix is compatible with multiplexing reactions, allowing additional exogenous or endogenous controls or targets to be run simultaneously for quality control or increased efficiency. Both the Mustang Purple™ passive reference dye-containing mix and the NO ROX mix can be used in conjunction with TaqMan* probes with FAM, VIC, ABY, and JUN dyes and QSY quenchers to provide detection of four targets in a single reaction.

TaqPath Bactopure Microbial Detection Master Mix

Applied Biosystems TaqPath BactoPure Microbial Detection Master Mix is optimized for low-level microbial DNA detection even in the presence of inhibitors from both purified samples and crude lysates.

Consistent low-level detection of bacterial, fungal, mammalian, and viral DNA, as well as antibiotic resistance markers Wide dynamic range* (up to 8 logs) enabling accurate detection on both low and high concentration samples Optimized for multiplexing—available in two formulations (one with and one without ROX passive reference) that enable from one- to four-target detection per reaction

TaqPath ProAmp Master Mix and TaqPath ProAmp Multiplex Master Mix

Applied Biosystems TaqPath ProAmp master mixes are designed to be high-performing, high-throughput genotyping and copy number variation (CNV) master mixes that work even with inhibited samples. They have the ability to detect up to four targets in one reaction, providing high specificity and reproducible genotyping and copy number variation determination. They have a wide dynamic range compatible with multiplexing applications,** and they exhibit tolerance of inhibitors commonly found in clinical samples prepared from human or animal sources (buccal swabs, blood, and card punches). All TaqPath ProAmp master mixes also feature 72-hour pre-PCR benchtop stability for automated workflows.

TaqPath 1-Step RT-qPCR Master Mixes for RNA and Virus Detection

TaqPath 1-Step RT-qPCR Master Mixes are 4X formulations designed for 1-step processing of RNA and DNA targets for pathogen detection, or for 1-step gene expression workflows. Versions with ROX passive reference dye, as well as versions designed specifically for multiplexing and formulated with either Mustang Purple passive reference dye or without ROX dye, are available.

TaqPath qPCR Master Mix, CG for DNA Detection

Applied Biosystems TaqPath qPCR Master Mix, CG is a 2X formulation designed for gene expression and miRNA analysis, containing a thermostable fast DNA polymerase and dNTPs in an optimized buffer solution for maximum robustness and reproducibility.

Ease of use

These formulations combine thermostable MMLV reverse transcriptase, dNTPs, and fast DNA polymerase all in a single tube, so all you have to do is add your sample, target-specific primers, and probes.


Optimized to tolerate inhibitors commonly found in clinical samples and to enable superior sensitivity, specificity, and dynamic range with multiplexing compatibility, TaqPath 1-Step RT-qPCR Master Mixes are an ideal solution for your most challenging applications.

Applied Biosystems by Thermo Fisher Scientific, qPCR