Suspension Culture Improves iPSC Expansion And Pluripotency Phenotype

Induced pluripotent stem cells (iPSCs) hold immense potential as a renewable source for various cell types, including islets, dopaminergic neurons, retinal cells, and cardiomyocytes. Yet. the widespread clinical use of these regenerative cell therapies depends on the cost-effective mass production of high-quality human iPSCs.

This study presents a novel protocol for iPSC expansion using 3D suspension culture within Vertical-Wheel® bioreactors. This modified 3D suspension culture method resulted in a nearly 100-fold expansion of iPSCs over five days, exceeding the growth observed in traditional 2D planar culture methods. Our findings demonstrate that 3D suspension culture not only improves the expansion capacity of iPSCs but also enhances their pluripotency phenotype. This method also results in the generation of more mature teratomas with fewer proliferative cells, indicating improved safety and efficacy.

Download the full white paper to learn more about the advantages of 3D suspension culture for iPSC expansion and the potential implications for regenerative medicine.