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Pfast™ - Fast Protein Expression Feasibility Analysis

Accelerate protein expression feasibility. Test the power of the Pfenex Expression Technology® platform with Pfast™ for actionable insights to advance your production requirements.

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What is Pfast?

Pfast™ - Fast Protein Expression Feasibility Analysis

Starting at $25,000, our Pfast™ Feasibility program delivers rapid insights into your protein's potential with the Pfenex platform. De-risk your development pipeline quickly and cost-effectively with ought a full-scale commitment.

How is Pfast different from a full Pfenex program?

Our advanced Pfenex strain engineering program is historically used for extremely challenging proteins or for identified lead candidates where expression optimization is needed. A full platform screen and scale-up is typically 10 to 12 weeks. Pfast accelerates this process using known high-potential toolbox subsets to quickly drive to the optimal expression strategy, or the final strain itself.

The Pfast Workflow

Experience the simplicity and efficiency of Pfast:

Contact Us: Speak with a Primrose scientist about how Pfast can empower you (CDA not required for initial discussion).

Easy Master Services Agreement: Contracting for Pfast programs is fast and low risk.

High-Throughput Screening: Evaluate 200 vector combinations in one high-performing strain from our vast Pfenex toolbox.

Actionable Path Forward: Receive a comprehensive report detailing results with recommendations for further strain and/or process development.

PFAST Feasibility Workflow:

Days 1–2

Pooled Cloning of single gene

  • 25–200 plasmid pool ligated with gene of interest
  • Transform and generate pooled seed culture

Days 3-6

Plate Pooled Culture

  • Automated 384 place selective medium plate
  • Cherry-pick to 96w growth plate

Days 7-8

HTP Expression

  • Grow and induce
  • Lyse, rank titer by CGE or ELISA
  • ID wells for affinity purification

Days 9-10

HIT Enrichment

  • Bead-based HTP IMAC
  • CGE: concentration and purity
  • Sequence (as needed)

Program Deliverables:

  • Detailed protein titer and analysis of soluble and insoluble fractions via SDS-CGE or ELISA.
  • Analytical assessments via Western blot and/or BLI, a multitude chromatographic method, and peptide mapping, provided at additional costs.
  • Sample material for further testing provided if suitable titers achieved.
  • Strategic recommendations for additional strain improvements, or advancement to 2L scale fermentation.

After your Pfast evaluation has completed, further leveraging the Pfenex platform and our know-how is available.

Further Strain Optimization

Results guide how to refine improved expression across multiple facets of additional strain engineering or advancement of the identified clone.

Scale-Up

Transition to 2L and then 30L, or 100L up and downstream processes development, including analytics. All done in house.

Flexible Licensing

Secure access to your new Pfenex strain via our industry-friendly licensing model, royalty and royalty-free options.

Primrose Bio