Nanopede Optical Filters
The Nanopede family includes fifty-one versatile, cost-effective filters covering the near UV and visible spectrum in 20 nm FWHM steps for Flight 1 and Flight 2, and 30 nm FWHM for NIR.
Flow cytometry interrogates cells through scattered light measurements and fluorescent markers. In spectral flow cytometry, the fluorescence is collected across the entire spectrum using discrete, back-to-back (spectrally adjacent) optical filters. Then the photons are binned such that spectral unmixing can resolve which fluorescent markers are present in the interrogated cell. As such, spectral flow cytometry requires filters covering the UV, visible, and NIR in discrete steps, which may lead to a desire to balance instrument cost and optical filter performance.
These essential performance and cost-effective filters cover the near UV and visible spectrum in even (Flight 1) and odd (Flight 2) 20 nm Full-Width, Half-Max (FWHM) steps. Moving into the NIR, the FWHM increases to 30 nm. The fifty-one filters in the Nanopede family have been designed with your application in mind.
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Features
- Coverage of the spectrum in discrete steps so you can test several configurations and define the best combinations possible.
- ODavg > 5 blocking from 330 to 900 nm, so you can optimize for the blocking depth and range you need.
- ODabs > 5 at key laser wavelengths to assist in reducing light source bleedthrough.
- Hard coated for consistent performance with Semrock quality for spectral repeatability and proven reliability for your most demanding applications.
- Availability in 12.5 mm housed standard size filters with 10 mm clear aperture, providing a compact filter package. (Custom sizing is also available to fit your unique instrument footprint.)
- Available now to support your immediate prototyping needs through future volume production requirements.
The Nanopede filter family provides a value approach to optical performance for your application and marks the beginning of a new generation of Semrock optical filters to match the rapidly developing and innovative market. Our understanding of essential filter performance is why we offer an off-the-shelf filter family designed for versatility with a contained cost. Partner with us on custom optical filters to meet your specific application needs.