microViscoStar® Online Differential Viscometer For UHPLC
A highly sensitive, on-line differential viscometer used with SEC-MALS-IV to determine the size and conformation of polymers, proteins and peptides separated by UHPLC.
Multiple Novel Technologies
The microViscoStar® incorporates multiple novel technologies to provide the highest sensitivity, stability and solvent compatibility of any available viscometer for UHPLC. Its ease-of-use and serviceability make it the perfect companion for Wyatt's microDAWN® light scattering and microOptilab™ refractive index detectors for UHPLC.
APC and UHPLC Applications
View the webinar Development of a New Online Micro-Viscometer for APC and UHPLC Applications.
To learn about the technological breakthroughs implemented in the microViscoStar, download ViscoStar: Innovations in Online Viscometry for GPC.
Another path to polymer and protein characterization: SEC-MALS-IV
When coupled with UHPLC size-exclusion chromatography and a compatible concentration detector such as the Optilab®, the microViscoStar derives the intrinsic viscosity (IV) of polymers, rapidly and with minimal sample and solvent consumption. An inherently valuable physico-chemical property in itself, IV is best utilized together with molar mass information from a microDAWN multi-angle light scattering (SEC-MALS-IV) to derive the hydrodynamic radius, conformation, and branching ratio of a macromolecule in solution.
Key benefits of the microViscoStar
- High sensitivity, equivalent to 5 ng of 100 kDa polystyrene in THF at 1.0 mL/min injected onto a standard GPC column
- High dynamic range of over 135,000:1. The full measurement range corresponds to over 13 mg/mL of 100 kDa polystyrene in THF
- Extended solvent compatibility with all-316 stainless steel transducers
- Temperature range from 4 °C to 70 °C for polymers or proteins that require a range of temperatures
- Minimal band broadening thanks to fast pressure transducers
- Pump pulse suppression to below 4 Pa (hardware alone) or 1 Pa (hardware + software) without loss of chromatographic resolution
- Automated capillary bridge tuning with no moving parts, to guarantee a perfectly balanced bridge, every run
- Enhanced thermal stability with drift of <1.25 Pa/hour