Epigenetics Application Guide: RNA Modifications

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RNA modifications have emerged as pivotal regulators of gene expression, influencing RNA stability, structure, splicing, and translation. With over 100 known chemical modifications across various RNA types, the field is rapidly expanding. In this white paper, we explore advanced techniques for RNA modification detection and sequencing, including RNA immunoprecipitation (RIP), cross-linking immunoprecipitation (CLIP), and miCLIP. Discover how these methods shed light on RNA-protein interactions and modification mapping.

Additionally, learn about the gold standard for quantifying RNA modifications and assessing antibody specificity: liquid chromatography tandem-mass spectrometry (LC/MS-MS) and valuable insights into optimizing these techniques, from antibody selection to ensuring accurate results through rigorous controls. With the increasing recognition of RNA's multifaceted role, these essential protocols and best practices for advancing their RNA modification studies are key to understanding RNA’s role in cellular processes.