Cell Line Engineering Services

Start your custom cell line engineering service project today

Whether you need pin-point single base edits with negligible off-target activity, large insertions at a safe harbor site, or other knock-out or knock-in edits, our exclusive technologies and experienced gene editing scientists will deliver more than you may have thought possible.

Choose from our different genome editing platforms or ask our scientists to customize a project to achieve your goals. We’ll use our patented and proprietary technologies, such as our Rapid Automated Cell Line Editing (RACE™) protocol, to ensure quality, accuracy, and fast turnaround times.

Take a look at what we can engineer for you

Here are just a few of the ways we can customize a mammalian cell line for your needs. If you don’t see what you’re looking for, simply contact us, we’ll get it done!

Capabilities

• Knock-out
• Knock-in (up to 20 kb)
• Point mutation or single-base editing
• Complex, multi-step cell line engineering
• Footprint-free edits
• TARGATT™ technology
• S-SELeCT™ technology
• CRISPR/Cas9 and variants
• MAD7

Cell types*

• iPSCs (healthy or diseased)
• HEK293
• CHO
• Custom
• Research grade
• cGMP grade
• Matched research and cGMP grade

Applications

• Disease modeling
• Tissue engineering
• Regenerative medicine R&D
• Allogeneic and autologous R&D
• Basic research
• Library construction
• Mammalian display
• Cell product cGMP manufacturing
• Cancer cell line editing
• T cell engineering

*We also offer CRISPR editing to generate rodent animal models — contact us to learn more.

NeonGreen-labeled titin protein in beating, hiPSC-derived cardiomyocytes.

We used CRISPR/Cas9 to label titin, a sarcomere protein, with the NeonGreen protein in hiPSCs, and then differentiated the hiPSCs into cardiomyocytes.

Choose our experience to maximize your success

With over 1,800 unique cell line models engineered by the scientists and technology developers at Applied StemCell, you can be confident in the quality and reliability of our service.

Fast turnaround times

As quick as 6 – 8 weeks when you select one of the Applied StemCell control lines or master cell lines, or 2 – 3 months if you send us your cell line

Customizable deliverables

Choose homozygous mutations, heterozygous mutations, footprint-free genome editing (ideal for GMP applications), or other customizations

High success rates

Over 98% projects completed to our customers' specifications

Supply chain security

All projects completed at our facilities in California (cell-based projects) and Oklahoma (animal projects)

See how our cell line engineering service works

1. Tell us what you need

Your dedicated business development manager will walk through your project requirements and goals with you, arranging a web call with our technical team, if needed.

2, Approve the strategy

Work with our technical team on the cell line engineering strategy, timelines, and milestones. We customize each project for your needs and do as much, or as little, of the plasmid, gRNA, or other reagent generation you need. We can also customize cell line characterization using a full range of technologies.

3. Send us your cells

Send in any materials you are supplying for the project, and we'll get to work.

4. Stay in touch

We'll begin your project and provide updates as agreed on. We pride ourselves on transparency, integrity, and will clearly communicate the status of your project.

5. Get your cell line

In as little as 6-8 weeks, if you choose one of our in-house cell lines, we'll be ready to send you your engineered cells.