August Bioservices has the seasoned expertise and a cutting edge and comprehensive toolbox of binding technologies to help you chart the best course for your drug discovery exploration. Starting with the right tool – to ascertain a quick and decisive “go/no go” decision – is crucial in saving you time and money in the drug development process.
These five case studies is your Binding Technologies Toolbox, covering the topics of Temperature Related Intensity Change (TRIC), Surface Plasmon Resonance (SPR), Differential Scanning Fluorimetry (DSF), and Isothermal Titration Calorimetry (ITC).
TRIC is the state-of-the-art technology that provides steady-state binding affinity (KD) in the millimolar-picomolar range in an immobilization-free environment. This rapid and reliable high-throughput screening approach requires a fluorescently labeled target sensitive to the binding event and measures the change in change in fluorescence upon heating as a function of time.
Surface Plasmon Resonance (SPR) is a proven label-free, in vitro tool that measures in real-time a variety of biomolecular interactions.
Differential Scanning Fluorimetry (DSF) requires no immobilization or labelling steps, as interactions can be evaluated in solution, giving way to quick assay set-up and optimization.
Isothermal Titration Calorimetry (ITC) is a powerful biophysical characterization method that provides direct measurement of the heat of a biomolecular interaction and delivers label-free measurement of H, G, -T S, KD, and stoichiometry in a single experiment.